Changes in bulk histone acetylation and cell cycle progression induced by HDACi.

<p>(<b>A</b>) Western blots, and (<b>B</b>) quantification of the changes induced in H3K9ac, H4K8ac, H4K16ac abundance upon VPA treatment (5 mM, 8 hours). (<b>C</b>) Triton-acid-urea gel of histones isolated from control (C), or VPA–treated cells over the same time course. Histone H4 associated with none, one or more acetyl residues are indicated. (<b>D</b>) <i>HDACi treatment impacts on the cell cycle.</i> Fluorescence activated cell sorting of untreated and VPA (5 mM), SAHA (2.5 µM) or TSA (165 nM) treated cells (8 hours). Cells associated with distinct stages of the cell cycle are indicated. Cells labelled Sub-G are either dead or apoptotic.