Analysis of tumor tissue obtained from mice at the end of the cutaneous carcinogenesis study.

(A) Representative Western blots showing increases in H3K9 acetylation in tumors treated with MS-275 as compared to vehicle. Tumor tissue was selected at random from 6 mice in each group (1 mouse from each cage). Mice were sacrificed and tissue was isolated 12–16 h after the last inhibitor dose. H3K9ac and total H3 blots were run separately on different gels using aliquots from the same sample preparations and processed in parallel. Uncropped Western blots are available as S5B Fig. (B) H3K9 acetylation levels were normalized to total H3 and quantified by densitometry using ImageJ (n = 3). (C) Average increase in histone H3K9 acetylation for MS-275 and vehicle treated groups (n = 6, unpaired t test, *p < 0.05).