Treatment with 5 Aza reduces Wnt7a methylation.

<p>A) 157 and 1299 cells were treated with 5 Aza for 2 hours and Wnt7a was measured by QPCR compared to untreated controls. Error bars are the SEM of triplicate experiments. B) 157 and 1299 cells were treated with 5 Aza for 1, 2, and 4 hours and compared to untreated cells. Wnt7a protein expression was measured by immunoblot with β-actin as a loading control. C) 157 and 1299 cells were transiently transfected with Fzd9 and PPRE luciferase and 48 hours after were treated with 5 Aza for 2 hours. Luciferase activity was measured and treated cells were compared to an empty control. Error bars are the SE of triplicate experiments. D) 1299 cells were treated with 5 Aza for 2 hours and percent methylation of the Wnt7a promoter was measured by the Methyl-Profiler system. Treated cells were compared to an untreated control. Error bar is the SE of triplicate experiments.