HIO-107 cells were treated with 5-aza-dC to examine changes in DNA methylation in CpG sequences in <i>hTREX84</i>.

<p><i>A,</i> HIO-107 cells were treated with 5-aza-dC at concentrations of 1, 5, 10, 50 µM respectively for 5 days. RT-PCR show <i>hTREX84</i> mRNA expression and <i>B</i>, western blot analysis show hTREX84 protein levels. <i>C, D,</i> Quantitative mRNA and Western blot data were calculated from densitometric analysis of bands with the NIH imageJ software, respectively. The values were normalized to β-actin as internal control.