Treatment with 5-Aza-dC upregulates the transcription of <i>PPP2R1A</i> in L02 cells.

<p>RNA was extracted from immortalized human normal hepatocyte L02 cells treated with or without 5-Aza-2′-deoxycytidine (5-Aza-dC), a DNA methylation inhibitor, for 48 hours at the indicated concentrations (2.5, 5.0 and 10.0 µM), and the <i>PPP2R1A</i> transcripts in the control group and the 5-Aza-dC treated group were quantified using real-time RT-PCR (normalized against <i>ACTB</i>). The results were normalized against the untreated control (Ctrl), which was given a value of as 1.0. Compared with the Ctrl, the levels of <i>PPP2R1A</i> mRNA in the 5-Aza-dC treated groups were increased by statistically significant levels (∗ <i>P</i><0.05). The level of <i>PPP2R1A</i> mRNA in the 10.0 µM 5-Aza-dC treatment group was significantly higher than the mRNA level in the 2.5 µM 5-Aza-dC treatment group (<b>^#</b><i>P<</i>0.05). The results represent the mean ± SD of at least three independent experiments performed in duplicate.