Comparison of Rtt109-Vps75 mediated acetylation (1-h incubation) on individual lysines on H3 from chicken erythrocytes, recombinant Xenopus histones, or recombinant yeast histones.

<p>Acetylation percentage of each site was determined by targeted MS-based method. The black and white bars depict the acetylation levels on recombinant Xenopus and yeast histones, respectively. The grey bar represents the acetylation levels on native chicken H3/H4 after 1 hour incubation with Rtt109-Vps75. The error bar represents the standard deviation in acetylation percentage from triplicate measurements. Note that the acetylation amount on both recombinant histones is initially negligible; however, there is ~25% H3K14ac and ~12% H3K23ac preexisting acetylation on chicken histones.