Surveyor analysis of the top 5 predicted off-targets for each sgRNA used. Panel shows amplified sequence of target and 3 representative off-targets, non-treated and treated with T7 endonuclease I. Digestion reveals genome edition (red asterisk) when the off-target sequence carries some mutations. Table shows overall results from all off-targets analyzed. No differences were observed in number of edited off-targets by IE-sgRNAs compared with SDE-sgRNAs (Chi-square test P = 0.751 n.s.).</p
<p>(A) For shRNAs, the magnitude of the off-target effect comparing the leave-one-out CSS to project...
Tables S2-S3: Next-generation sequencing (NGS; MiSeq) analysis of on-target editing evaluating delet...
CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functional...
(A) The target site (T) and ten predicted off-target sites (OT) for sgRNA91/Cas9. OT1~OT10 represent...
<p><b>(A, B)</b> Cumulative distribution of off-target transcripts grouped by their non-seed base-pa...
(A) Deletion and indel PCR screening. Representative clones exhibiting all possible band combination...
Genome editing with the CRISPR-Cas9 system has enabled unprecedented efficacy for reverse genetics a...
<p>(A) GGN18 and GGN20 sgRNAs and their genomic target sequences. Sequences of the variable regions ...
Additional file 3: sgRNA off-target analysis. Trp53, Rb1 and Rbl2 sgRNAs were analyzed for potential...
CRISPR/Cas9 genome editing technology is the frontier of life science research. They have been used ...
Schematic of the GFP activation assay for specificity analysis is shown on the top. A panel of sgRNA...
<p>List of off-target genes predicted by Picky for the bad siRNAs and four commercial siRNAs. Only d...
© The Author(s) 2018. Published by Oxford University Press. Motivation CRISPR/Cas9 is driving a broa...
The availability of genome sequences of numerous organisms and the revolution brought about by genom...
Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements...
<p>(A) For shRNAs, the magnitude of the off-target effect comparing the leave-one-out CSS to project...
Tables S2-S3: Next-generation sequencing (NGS; MiSeq) analysis of on-target editing evaluating delet...
CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functional...
(A) The target site (T) and ten predicted off-target sites (OT) for sgRNA91/Cas9. OT1~OT10 represent...
<p><b>(A, B)</b> Cumulative distribution of off-target transcripts grouped by their non-seed base-pa...
(A) Deletion and indel PCR screening. Representative clones exhibiting all possible band combination...
Genome editing with the CRISPR-Cas9 system has enabled unprecedented efficacy for reverse genetics a...
<p>(A) GGN18 and GGN20 sgRNAs and their genomic target sequences. Sequences of the variable regions ...
Additional file 3: sgRNA off-target analysis. Trp53, Rb1 and Rbl2 sgRNAs were analyzed for potential...
CRISPR/Cas9 genome editing technology is the frontier of life science research. They have been used ...
Schematic of the GFP activation assay for specificity analysis is shown on the top. A panel of sgRNA...
<p>List of off-target genes predicted by Picky for the bad siRNAs and four commercial siRNAs. Only d...
© The Author(s) 2018. Published by Oxford University Press. Motivation CRISPR/Cas9 is driving a broa...
The availability of genome sequences of numerous organisms and the revolution brought about by genom...
Pooled CRISPR-Cas9 screens are a powerful method for functionally characterizing regulatory elements...
<p>(A) For shRNAs, the magnitude of the off-target effect comparing the leave-one-out CSS to project...
Tables S2-S3: Next-generation sequencing (NGS; MiSeq) analysis of on-target editing evaluating delet...
CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functional...