Off-target site analysis.
- Zicong Xie (6082688)
- Daxin Pang (470601)
- Hongming Yuan (2200930)
- Huping Jiao (1846525)
- Chao Lu (4374)
- Kankan Wang (160564)
- Qiangbing Yang (6082691)
- Mengjing Li (6082694)
- Xue Chen (127892)
- Tingting Yu (419018)
- Xinrong Chen (6082697)
- Zhen Dai (819780)
- Yani Peng (5948021)
- Xiaochun Tang (4844148)
- Zhanjun Li (470605)
- Tiedong Wang (4559821)
- Huancheng Guo (404972)
- Li Li (14993)
- Changchun Tu (404976)
- Liangxue Lai (351702)
- Hongsheng Ouyang (470606)
Publication date
December 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) The target site (T) and ten predicted off-target sites (OT) for sgRNA91/Cas9. OT1~OT10 represents 10 potential off-target sites, and T represents target site. (B) T7E1 cleavage assays for the target site and the ten potential off-target sites; M, DL2000; (C) Sanger sequencing analyses of PCR amplicons that spanning the target sites. The cleavage sites are labeled with red arrow. (D) Sanger sequencing analyses of PCR amplicons that spanning the potential off-target sites. The potential cleavage sites are labeled with red arrow.</p
Add to ORKG