Decomposition by projection.

<p>(A) For shRNAs, the magnitude of the off-target effect comparing the leave-one-out CSS to projection. Pearson correlation coefficient = 0.43. (B) For individual shRNAs (top) and sgRNAs (bottom), in which the on-target magnitude passes FDR < 25%, distribution of on- and off-target magnitudes, as assessed by projection decomposition. (C) Scatter plots of on-target and off-target projection magnitudes for RNAi (top) and CRISPR (bottom) for all of the signatures of reagents in the dataset. While the 2 technologies show similar on-target activities, RNAi shows large off-target effects. CRISPR, clustered regularly interspaced short palindromic repeat; CSS, consensus seed signature; FDR, false discovery rate; RNAi, RNA interference; sgRNA, single guide RNA; shRNA, short hairpin RNA.