Analysis of off-target effects based on sequence similarity between an siRNA non-seed region and its corresponding target sequences.

<p><b>(A, B)</b> Cumulative distribution of off-target transcripts grouped by their non-seed base-pairing (Fig 4A; siVIM-270 off-target effects, 2AU (70), 1AU (52), None (27), 1GC (133), 2GC (151), Fig 4B; siVIM-805 off-target effects, 2AU (55), 1AU (52), None (20), 1GC (52), 2GC (46)). Off-target transcripts with more than 2AU or 2GC match were omitted due to their low number. <b>(C, D)</b> The average GC contents for non-seed region (positions 8–15) were calculated for each group of off-target transcripts. <b>(E, F)</b> The cumulative distribution of off-target transcripts of siVIM-270 with 1GC match (133 transcripts)(E) and 2GC matches (151 transcripts)(F), were sub-divided based on their GC contents at positions 8–15. ‘Low’ subgroups have GC content lower than the average while ‘High’ subgroups have GC content higher than the average.