A) Western blots of mouse CTL lysates from heterozygous or homozygous mice prepared and run on SDS–PAGE immediately (fresh lysate) or stored for 6 weeks at −20°C before analysis

The nitrocellulose filter was probed with anti-Slp2-a antibody, washed and reprobed with anti-Rab27a and anti-actin antibodies. WT, wild type. B) Western blots of human CTL lysates derived from healthy donors (lane 1) and patients lacking Munc13-4 (lane 2) or Rab27a (lane 3) probed with antibodies to Slp1 SHD, Slp2-a SHD or actin. Relative migration of molecular weight markers (in kiloDalton) is shown on the left.<p><b>Copyright information:</b></p><p>Taken from "Slp1 and Slp2-a Localize to the Plasma Membrane of CTL and Contribute to Secretion from the Immunological Synapse"</p><p></p><p>Traffic (Copenhagen, Denmark) 2008;9(4):446-457.</p><p>Published online 11 Feb 2008</p><p>PMCID:PMC2329822.</p><p>© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd