Immunoprecipitation and Western-blot analysis of DEL, KMH2 and L-428 cell lysates

23.1 (Panel A and C) and R24.1 (Panel B and D). Samples were separated on 4–20% Life-gel and transferred onto nitrocellulose membranes. The membranes were then blocked in 5% non-fat dry milk and probed with monoclonal antibody clones R23.1 (Panel A and B) and R24.1 (Panel C and D) followed by goat-anti-mouse IgG-AP. The membranes were developed in BCIP/NBT. A protein band of ~21 KDa as well as IgG light chain were detected in all three lysates examined. Lane 1 contains molecular weights and lanes 2–4 contain Immunoprecipitated samples of DEL, KMH2 and L-428.<p><b>Copyright information:</b></p><p>Taken from "Constitutive overexpression of a novel 21 kDa protein by Hodgkin Lymphoma and Aggressive Non-Hodgkin Lymphomas"</p><p>http://www.molecular-cancer.com/content/7/1/12</p><p>Molecular Cancer 2008;7():12-12.</p><p>Published online 24 Jan 2008</p><p>PMCID:PMC2267462.</p><p>