Detection of total LRP levels in oesophageal and breast cancer cells.

<p>(a) Detection of relative expression of the total 37 kDa LRP levels on protein lysates of various tumorigenic cell lines. Western blot analysis was performed using anti-LRP/LR specific primary antibody IgG1-iS18 and secondary FITC-coupled antibody on MCF-7, WHCO1 and MDA-MB 231 cells. β-actin was used as a loading control and 10 µg of protein lysates was loaded. Data are representative of three experiments carried out in triplicate using different cell cultures. (b) Bar chart of total LRP/LR levels of MCF-7, WHCO1 and MDA-MB 231 cell as analysed by Western blotting. Densitometry analysis was performed on the blots from Fig. 2a. WHCO1 and MDA-MB 231 cells displayed significantly higher total LRP/LR levels relative to MCF-7 cells. Data are representative of three experiments carried out in triplicate from different cell cultures.