A) Scheme to show the PEST sequence distribution in the 950 amino acid Slp2-a sequence encoded by full-length mouse Slp2-a (accession number: AB057754), indicating the SHD that binds Rab27a , C2A that binds phosphatidylserine (PS) and C2B domains

The sites where splicing variation within the linker have been reported are shown, 2S-I, 2S-II and 2S-III, as well as the content of four amino acids (Pro, Glu, Ser and Thr) in each domain and the number of clusters of PEST-like sequences identified by eye. B) COS-7 cell lysates expressing T7-tagged Slp1-5 treated with (+) or without (−) 100 ng/mL trypsin and probed with anti-T7 antibody. C) Cell lysates from melanocytes expressing Slp2-a treated with calpastatin peptide or calpain inhibitor in the presence of calcium (+) and probed with anti-Slp2-a or anti-actin antibody. Relative migration of molecular weight markers (in kiloDalton) is shown on the left.<p><b>Copyright information:</b></p><p>Taken from "Slp1 and Slp2-a Localize to the Plasma Membrane of CTL and Contribute to Secretion from the Immunological Synapse"</p><p></p><p>Traffic (Copenhagen, Denmark) 2008;9(4):446-457.</p><p>Published online 11 Feb 2008</p><p>PMCID:PMC2329822.</p><p>© 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd