CTL activity against the minor histocompatibility antigens (HY) and the LCMV peptide epitope gp33 was analyzed in vivo

(A and B) For analysis of HY-specific CTLs, female and male spleen cells (10 each) labeled with different amounts of CFSE were injected into the indicated mice, and 18 h later the ratio between both populations in the spleen was determined by flow cytometry. Immunization was performed by a single i.p. injection of 5 × 10 male C57BL/6 spleen cells 2 wk before the assay. The percentage of specific killing of male cells is indicated. One representative example per experimental group is shown in A, and all data are shown in B (horizontal bars indicate mean values). Serum anti-Tag antibody titers (mg/ml) are given for tumor-bearing mice. Genotype (B6, C57BL/6; Tg, LoxP-Tag), age, cancer status (−, no tumor; LTB), treatment (N/−, naive; I/+, immunized), and gender of mice (f, female; m, male) are indicated. Experimental groups were young naive female C57BL/6 mice (B6; 2–3 mo old; = 5), young immunized male C57BL/6 mice (B6; 2–3 mo old; = 3), young immunized female LoxP-Tag mice (Tg; 2–3 mo old; = 7), old immunized female C57BL/6 mice (B6; 15 mo old; = 4), and LTB female LoxP-Tag mice (Tg; 7–19 mo old; = 6). n.a., not analyzed. (C and D) For analysis of LCMV-gp33 peptide–specific CTLs, nonloaded and LCMV-gp33 peptide–loaded spleen cells (10 each) labeled with different amounts of CFSE were injected into the indicated mice, and 18 h later the ratio between both populations was determined by flow cytometry. Mice were immunized by a single s.c. injection of 10 cells of tumor line MC57-gp33-Hi 8–10 d before the assay. The percentage of specific killing of peptide loaded cells is indicated. Representative examples are shown in C, and all data are shown in D (horizontal bars indicate mean values). Genotype (B6, C57BL/6; Tg, LoxP-Tag), age, cancer status (−, no tumor; LTB), and treatment (N/−, naive; I/+, immunized) are indicated. Experimental groups were young naive C57BL/6 mice (B6; 2–3 mo old; = 5), young immunized C57BL/6 mice (B6; 2–3 mo old; = 6), and LTB LoxP-Tag mice (Tg; 19 mo old; = 4).<p><b>Copyright information:</b></p><p>Taken from "Immunogenicity of premalignant lesions is the primary cause of general cytotoxic T lymphocyte unresponsiveness"</p><p></p><p>The Journal of Experimental Medicine 2008;205(7):1687-1700.</p><p>Published online 7 Jul 2008</p><p>PMCID:PMC2442645.</p><p>