Cultured in either growth media (GM) or differentiation media (DM) for 1, 2, 3, or 4 days. Kindlin-2 levels were compared to an early (myogenin) and a late (myosin heavy chain; MHC) marker of differentiation. GAPDH was used as a loading control. Kindlin-2 expression was induced upon differentiation, with a peak expression at day 3 post-differentiation. Of note, the GM cell extract was from confluent cells, and confluence stimulates a small amount of differentiation.<p><b>Copyright information:</b></p><p>Taken from "Kindlin-2 is required for myocyte elongation and is essential for myogenesis"</p><p>http://www.biomedcentral.com/1471-2121/9/36</p><p>BMC Cell Biology 2008;9():36-36.</p><p>Published online 8 Jul 2008</p><p>PMCID:PMC2478659.</p><...
Kindlin-2 is a multidomain intracellular protein that can be recruited to beta-integrin domains to a...
<p>(A) Proteins were extracted from the cells at different stages, and WB assays were performed usin...
<p>(A) Control or Kindlin 2 siRNA was transfected into C2C12 cells. After 24 hr, C2C12 cells were in...
Ultured in either growth media (GM) or differentiation media (DM) for 1, 2, 3, or 4 days. Kindlin-2 ...
Ia (GM) or changed to Differentiation Media for two days (DM D2). Cellular area was determined using...
Nti-β1 integrin antibodies on undifferentiated (A; GM) C2C12 cells and C2C12 cells induced to differ...
Entiated C2C12 cells as determined by qPCR. Each value was calculated from 3 independent transfectio...
<p>(A) C2C12 cells were cultured in growth medium (GM) or differentiation medium (DM) for 0, 1, 3, 5...
Ectin coated cell culture dishes and incubated for 1 hour at 370C. Cells were fixed and then examine...
In differentiation media for 4 days, and then immunostained with anti-myosin heavy chain. Note that ...
Nti-paxillin (red) on C2C12 cells transfected with RNAi and then changed to differentiation media fo...
<p>(A) The efficacy of Kindlin 2 siRNA was detected by Western blot. (B) Control or Kindlin 2 siRNA ...
<p>(A) Immunofluorescence staining was performed in GM cells and DM cells (day 3) with or without Ki...
<p>(A) Control or Kindlin 2 siRNA was transfected into C2C12 cells. After 24 hr, C2C12 cells were in...
Abstract Background Integrins are required for normal muscle differentiation and disruptions in inte...
Kindlin-2 is a multidomain intracellular protein that can be recruited to beta-integrin domains to a...
<p>(A) Proteins were extracted from the cells at different stages, and WB assays were performed usin...
<p>(A) Control or Kindlin 2 siRNA was transfected into C2C12 cells. After 24 hr, C2C12 cells were in...
Ultured in either growth media (GM) or differentiation media (DM) for 1, 2, 3, or 4 days. Kindlin-2 ...
Ia (GM) or changed to Differentiation Media for two days (DM D2). Cellular area was determined using...
Nti-β1 integrin antibodies on undifferentiated (A; GM) C2C12 cells and C2C12 cells induced to differ...
Entiated C2C12 cells as determined by qPCR. Each value was calculated from 3 independent transfectio...
<p>(A) C2C12 cells were cultured in growth medium (GM) or differentiation medium (DM) for 0, 1, 3, 5...
Ectin coated cell culture dishes and incubated for 1 hour at 370C. Cells were fixed and then examine...
In differentiation media for 4 days, and then immunostained with anti-myosin heavy chain. Note that ...
Nti-paxillin (red) on C2C12 cells transfected with RNAi and then changed to differentiation media fo...
<p>(A) The efficacy of Kindlin 2 siRNA was detected by Western blot. (B) Control or Kindlin 2 siRNA ...
<p>(A) Immunofluorescence staining was performed in GM cells and DM cells (day 3) with or without Ki...
<p>(A) Control or Kindlin 2 siRNA was transfected into C2C12 cells. After 24 hr, C2C12 cells were in...
Abstract Background Integrins are required for normal muscle differentiation and disruptions in inte...
Kindlin-2 is a multidomain intracellular protein that can be recruited to beta-integrin domains to a...
<p>(A) Proteins were extracted from the cells at different stages, and WB assays were performed usin...
<p>(A) Control or Kindlin 2 siRNA was transfected into C2C12 cells. After 24 hr, C2C12 cells were in...