Kindlin 2 forms a complex with active β-catenin and TCF4.

<p>(A) Control or Kindlin 2 siRNA was transfected into C2C12 cells. After 24 hr, C2C12 cells were induced myogenic differentiation. At different time points, WB analysis was performed. (B) Protein bands in A were scanned, and relative band intensities were normalized for each β-actin band. The column diagrams represent average relative band intensity with standard error from three independent experiments. (C–E) Nuclear lysates from C2C12 cells (DM day 3) were prepared and then anti-TCF4 (C), anti-Kindlin 2 (D) or anti-active β-catenin (E) antibodies were used for Co-IP assays. (F) Immunofluorescence staining for Kindlin 2 (Alexa Flour 488, green) or TCF4 (Alexa Flour 568, red) was performed in C2C12 cells (DM day 3).