Kindlin-2 is required for myocyte elongation and is essential for myogenesis-3

Ia (GM) or changed to Differentiation Media for two days (DM D2). Cellular area was determined using Metamorph on immunostained cells overexposed to reveal their cell borders. There was a small but significant difference between conditions in undifferentiated cells (kindlin-2 RNAi vs. scrambled RNAi: 7692 +/- 172 pixels v 9080 +/- 348 pixels, n= 240 cells, p = 0.006). There was a substantial difference between conditions in differentiated cells (kindlin-2 RNAi vs. scrambled RNAi: 7510 +/- 599 pixels v 13180 +/- 906 pixels, n = 270, p = 0.001). B. C2C12 cells at differentiation day 2 immunostained with anti-kindlin-2. The photomicrographs emphasize the obvious size difference between scrambled RNAi transfected cells and those transfected with kindlin-2 RNAi. They also reinforce the successful knockdown of kindlin-2 protein by RNAi. (scale bar = 20 μm).<p><b>Copyright information:</b></p><p>Taken from "Kindlin-2 is required for myocyte elongation and is essential for myogenesis"</p><p>http://www.biomedcentral.com/1471-2121/9/36</p><p>BMC Cell Biology 2008;9():36-36.</p><p>Published online 8 Jul 2008</p><p>PMCID:PMC2478659.</p><p>