Cell cycle regulation of IFT proteins.

<p>(A) Top panel is Coomassie Blue (CB) stained gel of total protein from indicated times. Equal protein was loaded in each lane. The last lane contains molecular weight markers. Bottom panels show Western blots from the same set of samples using indicated antisera. (B) Top panel is Coomassie Blue stained gel loaded with equal cell number per lane. The amount loaded was not recalibrated after mitosis and represents equal mother cell equivalents. Bottom panels show Western blots from the same set of samples using indicated antisera. (C,D) Quantitation of Western signals from (A,B) respectively. CB staining was used to normalize in (C), while HSP70B signal was used to normalize in (D). Normalized data were plotted relative to the signal at 0 hrs that was set to a value of 1. (E) Fraction of flagellated cells as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030729#pone-0030729-g001" target="_blank">Fig. 1B</a>.