Cyclin A, Cyclin B1and Cdc2 evaluation by western blot.

<p>(A)Total lysates of cells exposed to 10 (HL60) and 20 μM (U937, KG1a and Jurkat) T44Bf for 15 and 17h were analyzed for Cyclin A by Western blot. Densitometry for Cyclin A blot was performed using Image J software. Arbitrary units represent protein level analysis respect to load control β-actin. (B) Cells were exposed to 10 (HL60) and 20 μM (U937, KG1a and Jurkat) T44Bf for 15h. Then, the drug-containing medium was replaced by fresh culture medium for 2 and 5h. Total cell lysates were analyzed by Western blot for Cyclin B1 and Cdc2. Densitometry for Cyclin B1 and Cdc2 blots were performed using Image J software. Arbitrary units represent protein level analysis respect to load control α-tubulin. *P < 0.05.