Western blot analysis for E-Cadherin and antiapoptotic Bcl-2 proteins in CRC cells treated with Obatoclax.

<p>(<b>A</b>) Western blotting for E-Cadherin in four CRC cell lines after 24 h treatment with Obatoclax in escalating doses (left). Corresponding densitometric analysis relative to untreated controls and adjusted to Tubulin as loading control. (<b>B</b>) Western blotting for Mcl-1, Bcl-2 and Bcl-x_L in HT29 cells (left) and SW480 cells (right) after 24 h treatment with Obatoclax. Tubulin served as a loading control. Western blots are representative for at least three blots from independent experiments.