Cytometry and quantitative Western blots of standards.

<p>(A) Left 4 lanes are recombinant cyclin B1 at two-fold dilutions. Recombinant cyclin B1 and lysate concentrations were adjusted so that the band intensities for recombinant cyclin B1 spanned the range of those of the lysates. Trypsin inhibitor was added to each sample to bring the total protein per lane to 50 µg. (B) Lyophilized standard cell preparations from the same samples as used for Western blot were stained for cyclin B1 with GNS1-Alexa Fluor 647 (A647) and DNA (DAPI). Arrows indicate the mean cyclin B1 fluorescence. Background fluorescence was subtracted and data were plotted as a hyperlog transformation which allows plotting of negative and zero values on a linear lower section (−10 to 10) and higher values (>10) logarithmically <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007064#pone.0007064-Bagwell1" target="_blank">[22]</a>.