<p>The Western blots from the 4 NP patients and the 4 CFNP patients used for proteomic experiments are shown with their respective loading controls. <b>A</b>: Each protein is analyzed in three technical replicates. For normalization, the acrylamide gels were stained with Coomassie blue (total migrated protein detection) just after electro-transfer and scanned using the Odyssey LI-COR. Using the Odyssey software, protein intensity values were normalized with their corresponding loading control intensity values. <b>B</b>: Box plots of the intensity calculated with the Odyssey software, *p<0.01, Mann and Whitney test. (a.u.: arbitrary units). We report Maximum (max), minimum (min), first quartile (Q1), third quartile (Q3).</p
<p>Immunoblot results are from a representative experiment with β-actin as loading control. The dens...
<p>Western blots of membrane proteins prepared from wild-type (WT) and ciprofloxacin-resistant (CIP-...
<p>Six proteins were selected. Proteins were separated on a 12% SDS-PAGE gel and immunoblotted with ...
<p>(A) The proteins of interest were analyzed using 50 µg of protein extracts from fibroblast, f-rES...
<p>Protein extracts from IF, NIF, or NF samples (40 µg) were subjected to polyacrylamide gel electro...
Western blotting has been a key technique for determining the relative expression of proteins within...
<p>The coefficient of variation of four proteins with various dispersion levels was calculated by we...
<p>The graphs next to them illustrate the relative protein densities. <b>A</b>–<b>H</b> Blots of hea...
(A) Top: membranes used in Fig 3A stained with PonceauS, in triplicates. Bottom: Western Blot signal...
<p>A. Representative western blots of target proteins and β-actin, the standard. At least two gels w...
<p>A and B. Coomasie blue stained gel. Lane M in A and B are protein molecular weight markers and th...
<p>Bars represent normalized (vs GAPDH) protein levels (Mean ± S.E.M.). Symbols denote statistically...
<p>Top, Representative immunoblots of each protein and GAPDH, as a loading control. Bottom, Bar grap...
<p>A. Western blot, the raw data used for calculating protein expression measured by immunoreactive ...
<p>A) Representative Western blot of protein extracts (20 µg) from a pool of 8 control testes or tes...
<p>Immunoblot results are from a representative experiment with β-actin as loading control. The dens...
<p>Western blots of membrane proteins prepared from wild-type (WT) and ciprofloxacin-resistant (CIP-...
<p>Six proteins were selected. Proteins were separated on a 12% SDS-PAGE gel and immunoblotted with ...
<p>(A) The proteins of interest were analyzed using 50 µg of protein extracts from fibroblast, f-rES...
<p>Protein extracts from IF, NIF, or NF samples (40 µg) were subjected to polyacrylamide gel electro...
Western blotting has been a key technique for determining the relative expression of proteins within...
<p>The coefficient of variation of four proteins with various dispersion levels was calculated by we...
<p>The graphs next to them illustrate the relative protein densities. <b>A</b>–<b>H</b> Blots of hea...
(A) Top: membranes used in Fig 3A stained with PonceauS, in triplicates. Bottom: Western Blot signal...
<p>A. Representative western blots of target proteins and β-actin, the standard. At least two gels w...
<p>A and B. Coomasie blue stained gel. Lane M in A and B are protein molecular weight markers and th...
<p>Bars represent normalized (vs GAPDH) protein levels (Mean ± S.E.M.). Symbols denote statistically...
<p>Top, Representative immunoblots of each protein and GAPDH, as a loading control. Bottom, Bar grap...
<p>A. Western blot, the raw data used for calculating protein expression measured by immunoreactive ...
<p>A) Representative Western blot of protein extracts (20 µg) from a pool of 8 control testes or tes...
<p>Immunoblot results are from a representative experiment with β-actin as loading control. The dens...
<p>Western blots of membrane proteins prepared from wild-type (WT) and ciprofloxacin-resistant (CIP-...
<p>Six proteins were selected. Proteins were separated on a 12% SDS-PAGE gel and immunoblotted with ...