Expression and localization of <i>spe-44</i> mRNA.

<p>A. Quantification of <i>spe-44</i> expression by qRT-PCR. Transcript levels in each sample were normalized against actin as an internal control; the scale was arbitrarily set at one for the <i>fem-1</i> L3 value. Mean values and standard deviations are from triplicate samples of the indicated stage and genotype. ND, not determined. Genotypes from left to right are wild-type hermaphrodites, wild-type males, <i>fem-3(gf)</i> hermaphrodites, <i>fem-1(lf)</i> hermaphrodites, and <i>fog-1(lf)</i> hermaphrodites. B. <i>In situ</i> hybridization for <i>spe-44</i> transcript (arrow) in dissected <i>fem-3(gf)</i> L3 gonad. C. Same gonad stained with DAPI. D. <i>In situ</i> hybridization for <i>spe-44</i> in dissected <i>fem-1(lf)</i> L4 gonad.