Pattern of SPE-44 protein expression and localization.

<p>A. Wild-type adult male gonad co-stained with anti-SPE-44 (green) and DAPI (red). Insert at higher magnification shows details of nuclear morphologies during the appearance/disappearance of SPE-44 labeling. Arrow indicates unlabeled DNA. B. Individual nuclei from DAPI-stained wild-type male gonads that were co-stained with anti-SPE-44 only (S44), anti-dimethylated histone H3 (Lys9) antibody only (H3K9), or both antibodies. Top row, DAPI; middle row, antibody (Ab); bottom row, merged image. C. Wild-type male gonad stained with anti-SPE-44 (green), anti-MSP (blue), and DAPI (red). D–F. Wild-type hermaphrodite gonads stained with anti-SPE-44 (green) and DAPI (red). D. L4 larval stage. E. Adult stage. F. Spermatogenesis to oogenesis transition at the L4/adult molting stage. Insert shows details of the first oocyte nuclei interspersed with a few last SPE-44-staining nuclei. The pachytene region (p) is indicated for orientation.