H3K9me2 labeling is reduced in serially passaged <i>smrc-1</i> mutants.

(A) H3K9me2 distribution as detected by immunolabeling in wildtype and smrc-1(om136) F2 worms raised at 25°C. Gonads were dissected at 18 hr post-L4 stage and labeled with anti-H3K9me2 antibody and counterstained with DAPI to visualize DNA. Panel show sets of pachytene nuclei. (B) H3K9me2 distribution as detected in wildtype and smrc-1(ea8) animals passaged for 30 generations at 25°C. Gonads were dissected at 18 hr post-L4 stage and labeled with anti-H3K9me2 antibody and counterstained with DAPI to visualize DNA. Each panel shows a set of pachytene nuclei from four different individuals with different staining intensities and/or patterns. Histograms indicate the corrected total nuclear fluorescence (CTNF, see Materials and methods) for a set of nuclei within randomly selected wildtype and smrc-1 F30 germ lines; asterisks indicate the germ lines included in the images. Number of gonads assayed: 4 wt and 21 smrc-1 males; 4 wt and 20 smrc-1 hermaphrodites. 6 germline nuclei assayed/gonad.