Expression patterns in 11 <i>GRAS</i> genes by real-time quantitative RT-PCR analysis.

<p>cDNAs from nine different tissues of eight developmental stages were used to detect the expression of eleven <i>GRAS</i> genes in which <i>ZmGRAS40</i>, <i>ZmGRAS54</i> and <i>ZmGRAS62</i> have multiple splice variants. The quantitative primers for <i>ZmGRAS40</i>, <i>ZmGRAS62</i> were used to test all of the variants, and the quantitative primers for the gene <i>ZmGRAS54</i> were used to test the main two transcripts (GRMZM2G144744-T01 and GRMZM2G144744-T02). The relative expression levels which were normalized to ACTIN were determined by the comparative CT method (2^−ΔΔCT) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185418#pone.0185418.ref062" target="_blank">62</a>] Three biological replicates were conducted for each experiment.