qRT-PCR validation of genes expressed in the developing ovary identified by microarray.

<p>qRT-PCR analysis of mRNA from isolated XX and XY gonads from 11.5, 12.5 and 13.5 dpc mouse embryos using gene-specific primers for <i>Slitrk1</i> (<b>A</b>), <i>Fam196b</i> (<b>B</b>), <i>D630039A03Rik</i> (<b>C</b>), <i>Tmem174</i> (<b>D</b>), <i>Lrrc34</i> (<b>E</b>), <i>Lypd6</i> (<b>F</b>), <i>Egfl6</i> (<b>G</b>), <i>Magi2</i> (<b>H</b>), <i>Dmrtc1c1</i> (<b>I</b>), <i>Smc1b</i> (<b>J</b>), <i>Spdya</i> (<b>K</b>), <i>D6Mm5e</i> (<b>L</b>), <i>Ccdc41</i> (<b>M</b>), <i>Foxl2</i> (<b>N</b>) and <i>Amh</i> (<b>O</b>) relative to <i>Sdha</i> (mean +SEM of at least three independent experiments; two-tailed, unpaired t-test; *p≤0.05, **p≤0.01, ***p≤0.001). Individual experiments were performed in triplicate on RNA obtained from pooled gonads from 3–4 littermates. All candidate genes were confirmed to be higher expressed in the ovary compared to testis at least at one of the developmental stages investigated. <i>Foxl2</i> served as control gene for ovary, <i>Amh</i> as control gene for testis samples.