Prospective validation of RNA-Seq analysis using an independent cohort of 12 patients by qRT-PCR.

<p>Twenty-seven up-regulated genes and 15 down-regulated genes were selected for validation. The fold changes of selected genes measured by qRT-PCR were statistically significant (<i>P</i><0.05). Gene expression difference was considered to be valid if the direction of change was the same (as estimated by RNA-Seq analysis). The percentage of concordance of qRT-PCR with the change of direction estimated by RNA-Seq analysis for the selected genes was 80%. *: The expression of GPC3 in CD90⁺NTSCs was not detected and its fold change could not be calculated. Further analysis by Fluidigm digital array confirmed the finding. **: The expression of BMPER in CD90⁺CSCs was not detected. Further analysis by Fludigim digital array confirmed the finding.