<i>MHC-II</i> expressing B cells have active histone modifications distributed across the <i>HLA-DRA</i> gene.

<p>(A) The <i>HLA-DRA</i> gene region and additional PCR amplicons (−2000 and +5800) used in this and subsequent experiments are depicted. (B) Using the indicated antibodies, the distribution of unmodified H3, CIITA, and modified histones at the <i>HLA-DRA</i> gene was analyzed by ChIP-qPCR for the amplicons described in A in <i>MHC-II</i> expressing (Raji, black) and non-expressing (RJ2.2.5, CIITA-deficient, grey; SJO, RFX5-deficient, clear) B cell lines. The data are plotted as the average of the percent input values from three biological replicates and error bars represent standard error. In the unmodified histone H3 panel, an asterisk (*) indicates a Student’s t-test value of p<0.05 comparing the −600 and −300/WXY region, and double asterisks (**) indicate a t-test value of p<0.01 between +300 and −300/WXY in Raji cells.