Histone methyltransferase MLL1 core complex proteins are recruited to <i>HLA-DRA in B cells.</i>

<p>MLL core complex proteins, MLL1, WDR5, ASH2L, RbBP5, and DPY-30 were assessed for their binding across the <i>HLA-DRA</i> gene in Raji (black), RJ2.2.5 (CIITA-deficient, grey), and SJO cells (RFX5-deficient, clear) by qPCR after DSG-ChIP. The negative control IgG ChIP pulldowns conducted concurrently with these experiments are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037554#pone-0037554-g003" target="_blank">Figure 3B</a>. The data represent the average of the percent input values from the pulldown of each indicated protein at each amplicon as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037554#pone-0037554-g002" target="_blank">Figure 2A</a>. The values are an average of 3–5 biological replicates and the error bars represent the standard error. For RFX5-dependent factors, the asterisks represent Student’s t-test values p<0.05 of statistical significance for comparisons between RJ2.2.5 and SJO. For CIITA-dependent factors, the asterisks represent Student’s t-test values p<0.05 between Raji and RJ2.2.5 cells.