GCN5 and PCAF containing histone acetyltransferases ATAC and STAGA are recruited to <i>HLA-DRA.</i>

<p>(A) Dual crosslinking ChIP-qPCRs for histone acetyltransferases GCN5, and PCAF were conducted for regions across <i>HLA-DRA</i> in Raji (black), RJ2.2.5 (CIITA null, grey), and SJO (RFX5 null, clear) cells as above. (B) Binding of the ATAC (ADA2a, ATAC2, YEATS2, NC2-β) and STAGA (ADA2b, TRRAP, TADA1L) complex subunits was determined as in A. The average of percent input values from the DSG-ChIP for each indicated protein is shown with the values averaged from 3–5 biological replicates. The asterisks indicate a Student’s t-test value of p<0.05 at each amplicon when compared to the control IgG values for each cell line.