GCN5 maintains histone acetylation at specific target genes and in a widespread manner in NSC.

<p>(A–C) ChIP assays were conducted on control and <i>GCN5</i> KO NSC lines at both N-Myc (A–B) and <i>GCN5</i> target genes (C). <i>GCN5</i> binding itself was undetectable at any target. “a” and “b” represent biological replicate sets of neurospheres isolated from littermate embryos. (D) Control and <i>GCN5</i> KO NSC were immunostained for pan-Ac-H4 (Red) and DAPI (Blue). Scale Bars are 100 µM.