Histone modifications are perturbed by loss of Msl2.

<p>(<b>A</b>) Representative immunoblot analysis of nuclear extracts prepared from wild-type, <i>Msl2^−/−</i>, and <i>Msl2</i>-rescue cell lines. Panels were probed with the antibodies indicated. (<b>B</b>) Quantification of (A). Mean expression levels of the various modifications in the cell lines were quantified and expressed relative to those in wild-type cells after normalization to H3 levels. Error bars represent standard deviation (n ≥3). (<b>C</b>) Q-PCR showing expression levels of the genes indicated. Expression levels in the cell lines are expressed relative to those in wild-type cells, following normalization to <i>Gapdh</i>.