Disturbed glucose homeostasis with elevated insulin sensitivity in <i>Mbd5^−/−</i> mice.

<p>(<b>A</b>) Blood glucose levels in WT, <i>Mbd5^+/−</i> and <i>Mbd5^−/−</i> mice in the fed state at the indicated ages. The numbers in brackets indicate the number of animals studied. (<b>B</b>) Blood glucose and serum insulin levels of 2-week-old mice in the fed state. For each group, n = 5. (<b>C</b>) The OGTT of control and Mbd5-knockout male mice at P14. For each group, n = 5. (<b>D</b>) The ITT of control and Mbd5-knockout male mice at P14. For each group, n = 5. *, P<0.05; **, P<0.01; ***, P<0.001. (<b>E</b>) Akt activation in the liver of control and Mbd5-knockout mice at P14. The protein levels were analyzed by western blotting with the antibodies indicated. α-tubulin was used as a loading control. (<b>F</b>) Alteration in the mRNA levels of glycolytic genes in the livers of Mbd5-knockout mice at P14. The expression of each gene was normalized to <i>β-actin</i>. At least 5 pairs of matched mutant and wild-type mice were used for comparison. (<b>G</b>) Lactate levels in the livers of Mbd5-knockout mice and controls at P14. n = 3 per genotype *, P<0.05; **, P<0.01; ***, P<0.001.