GIA synergies against the vaccine-heterologous FVO parasite clone.

<p>Percentage GIA against the FVO parasite clone over increasing gradients of concentrations of IgG from rabbits immunized with <b>A</b>) PfEBA175, <b>B</b>) PfRH2 or <b>C</b>) PfRH4 with (blue line) or without (solid black line) the addition of a fixed low concentration of PfRH5-immunized rabbit IgG (0.156 mg/mL) which, when used alone, gives approximately 20% GIA (dashed black line). Predicted additive effects were calculated according to Bliss independence (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002991#s4" target="_blank">Materials and Methods</a>) and illustrated as the red line on each graph. Data points represent the mean of triplicates from two independent experiments. Bars indicate SEM for all six replicates over two experiments. Asterisks indicate that the predicted and observed values differed significantly (*<i>P</i><0.05; ** <i>P</i><0.01; 2-way ANOVA with Bonferroni post-hoc testing).