Growth inhibitory activity of sera from immunized mice and rabbits.

<p>5–6 week old female BALB/c mice (<i>n</i> = 7) were immunized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020977#pone-0020977-g001" target="_blank">Figure 1</a>. Sera were obtained two weeks post boost (day 70) by terminal bleed and the IgG was purified from the pooled sera. The GIA activity of the serum was assessed by incubating the IgG at a final concentration of 5 mg/ml with <i>P. falciparum</i> cultures. The ELISA titers and the % growth inhibition of the pooled serum are shown against both the homologous 3D7 and heterologous FVO strains of PfAMA1 (A). New Zealand white rabbits (<i>n</i> = 8) were immunized as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020977#pone-0020977-g002" target="_blank">Figure 2</a>. Two weeks after the final vaccination the sera were obtained. The effect of the purified IgG on parasite growth from each individual rabbit was evaluated by incubating the IgG at a concentration of 6 mg/ml with mature <i>P. falciparum</i> schizonts. The % GIA values for individual rabbits and the median are shown against both the 3D7 and FCR3 strains at 6 mg/ml IgG concentration (B). There was no correlation between the 3D7 and FCR3 GIA (C). There was a significant positive correlation between the 3D7 ELISA titers and 3D7 GIA (R² = 0.68, *<i>P </i>≤0.05) and with FCR3 GIA (R² = 0.51, *<i>P </i>≤0.05) using Pearson's rank correlation (D).