3E2 antibody inhibits endothelial cell proliferation.

<p>HMEC-1 cells are tracked by videomicroscopy up to 24 h after treatment by 3E2 or isotypic control. A) Cell number per field in function of time. Histograms show the mean doubling time (n = 6 for IgM and n = 9 for 3E2, mean±SEM; *p<0.05). B) Microphotographs of representative fields of HMEC-1 in function of time. Magnification 10×. C) Number of mitosis summed every 6 h for 24 h (n = 5, mean±SEM; *p<0.05). D) Duration of the mitosis (n = 5, mean±SEM; *p<0.05). E) Cell death quantification detected by sub-G1 and hoechst assays from 3E2- or IgM-treated HMEC-1 (n = 3; mean±SD; ns: p>0.1).