3E2 antibody inhibits <i>ex vivo</i> angiogenesis.

<p>A) Viability measured by MTT of HMEC-1 treated for 24 h with increasing concentration of 3E2 (▾) or isotypic control (▪) (n = 3; mean±SEM; *p<0.05). B) Western blot of phosphor-ERK and phosphor-AKT from cells treated with either 40 µg/ml 3E2 or IgM-control antibody (n = 3). C) Photographs of sprouting vessels from aorta 5 d post-treatment by an increasing dose of 3E2 (n = 3). D) Sprouting index from aorta rings 5 d post-treatment with 3E2 or isotypic control (n = 3, mean±SEM; *p<0.05).