LGRs do not reduce cell numbers by inducing cell death and LGR 1404, 1406 and 1407 significantly reduce endothelial cell migration at 10 µM.

<p>A: HMEC-1 cells were seeded in the same density as for the proliferation assay and treated with the respective compound at 30 µM for 72 h to test whether reduced cell numbers in the proliferation assay are due to inhibition of proliferation or to excessive cell death. No increase of detached, obviously dead or propidium iodide positive cells was detected. B and C: Confluent layers of HUVECs were scratched and the cells were allowed to migrate for 16 h in the presence or absence of 10 µM of the compounds. B: The columns indicate the area re-covered by migrating cells (n = 3, mean ± SEM, * p<0.05, One Way ANOVA, Dunnett). C: Scratches at endpoint, representative images taken out of three experiments.