Mutation of <i>rpn-10</i> suppresses lethality and polarity defects in <i>math-33(tm3561); usp-47(RNAi)</i> embryos.

<p>(A) Embryonic lethality of <i>math-33(tm3561); usp-47(RNAi)</i> is reduced by either of two <i>rpn-10</i> mutations. Standard error of the mean is indicated by the error bars. <i>n</i>>350 embryos for N2 controls and <i>n</i>>600 for the other genotypes. Asterisks indicate significance compared to <i>math-33(tm3561)</i> single mutants, <i>p</i><0.01, Student's t-test (B) Data showing the suppression of phenotypic defects in early <i>math-33(tm3561);usp-47(RNAi)</i> embryos by <i>rpn-10</i> mutations. (C) Distance in micrometers of the centrosome from the embryo cortex when it is first detectable. <i>rpn-10</i> mutation suppresses the absence-of-pseudocleavage phenotype and the mislocalization of the centrosome compared to <i>math-33(tm3561); usp-47(RNAi)</i> controls. None of the 18 centrosomes observed in column 2 were detached from the paternal pronucleus, indicating that the detachment phenotype was also completely suppressed. Results in column 4 were significantly different in a Student's t-test <i>p</i><0.01 compared to column 1 controls. Data from columns 1, 3, and 4 are also displayed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003092#pgen-1003092-g005" target="_blank">Figure 5C</a>.