<i>elt-2</i> is required for the viability of the <i>rpn-10</i> mutant.

<p>(A) Treatment of the <i>rpn-10</i> mutant, but not wild-type animals with <i>elt-2</i> RNAi results in a developmental arrest phenotype. (B) The <i>rpn-10</i> mutant shows elevated expression of GFP::LGG-1 as shown by fluorescence microscopy or measurement of the fluorescence in the images (C) (n = 10 for each genotype, p<0.001 by <i>t</i>-test). (D) The <i>rpn-10</i> mutant also shows an increase in GFP::LGG-1 puncta formation in both the intestine and in the seam cells as shown by fluorescence microscopy. Puncta are indicated by arrows. (E) Scoring of puncta number in the seam cells shows a significant increase in puncta count in the <i>rpn-10</i> mutant (n = 41 for WT and = 24 for <i>rpn-10</i>, *** represents p<0.0001 by <i>t-</i>test). (F) Measurement of puncta number in the intestine also demonstrates a significant increase in puncta number in the <i>rpn-10</i> mutant (n = 14 for WT and = 13 for <i>rpn-10</i>, *** represents p = 0.0024 by <i>t</i>-test). (G) The inhibition of autophagy with <i>an atg-13</i> mutation delays the developmental time of an <i>rpn-10; atg-13</i> mutant compared to the <i>rpn-10</i> or <i>atg-13</i> mutants and only 80% of worms reach adulthood (n = 283 for WT, = 298 for <i>rpn-10</i>, = 282 for <i>atg-13</i>, and = 300 for <i>rpn-10; atg-13</i>, p<0.0001 for differences between <i>atg-13; rpn-10</i> and <i>rpn-10</i> at the 88 hour time point by Fisher’s exact test) (H) The inhibition of autophagy in <i>rpn-10</i> mutant worms expressing a Q44::YFP transgene in the intestine through treatment with <i>atg-13</i>, <i>atg-16</i>.<i>2</i>, or <i>prmt-1</i> RNAi treatment, starting on day 1 of adulthood, reduces the protective effect of the <i>rpn-10</i> mutation. Shown are the percentage of animals with aggregates when scored after 72 hours of RNAi treatment (for <i>atg-13</i> RNAi, n = 50, p = 0.0002 by Fisher’s exact test; for <i>atg-16</i>.<i>2</i> RNAi, n = 50, p = 0.003; and <i>prmt-1</i> RNAi, n = 50, NS).