The loss of <i>eif-3.K</i> results in reduced programmed cell deaths.

<p>(A) The inactivation of <i>eif-3.K</i> by RNAi or by genetic deletion reduced cell corpse numbers throughout embryogenesis. Cell corpses of the indicated genotype or RNAi treatment were scored at the comma, 1.5-fold, 2-fold, 3-fold and 4-fold embryonic stages. The <i>eif-3.K(gk126)</i> embryos were compared to the wild-type embryos, and the <i>eif-3.K(RNAi)</i> embryos were compared to control(RNAi) embryos at each stage. All comparisons were performed using the unpaired t test (*P<0.05, **P<0.001). Data are presented as the mean ± standard deviation. Error bars represent S.D. Greater than 20 embryos per stage were analyzed. (B) The loss of <i>eif-3.K</i> reduces TUNEL staining in <i>nuc-1</i> embryos. The number of cells exhibiting TUNEL staining was determined in embryos of the indicated genotypes at the 1.5-fold stage. The <i>eif-3.K(gk126)</i> embryos were compared to the wild-type embryos, and the <i>nuc-1(e1392)</i>single mutants were compared to the <i>eif-3.K(gk126); nuc-1(e1392)</i> double mutants. Comparisons were performed using the unpaired t test (*P<0.05, **P<0.001). Data are presented as the mean ± standard deviation. Error bars represent S.D. Greater than 20 embryos of each genotype were analyzed. n.s. indicates no significant difference. (C) A loss of <i>eif-3.K</i> reduces cell corpse number in the germline. Cell corpses in the germline of the <i>ced-1</i> single mutants (white columns) and <i>eif-3.K; ced-1</i> double mutants (black columns) were counted at the indicated times after entry into the adulthood. The y axis represents the average number of cell corpses scored in each gonadal arm. The <i>eif-3.K(gk126); ced-1(e1735)</i> double mutants were compared to the <i>ced-1(e1735)</i> single mutants at the same developmental stage using the unpaired t test (*P<0.05, **P<0.001). Data are presented as the mean ± standard deviation for >20 gonadal arms. Error bars represent S.D. (D) A loss of <i>eif-3.K</i> increases the number of extra surviving cells in weak <i>ced-3</i> mutants. Cells that failed to undergo programmed cell deaths in the anterior pharynx were scored in the indicated animals. All comparisons were performed using the unpaired t test (*P<0.05, **P<0.001). Data are presented as the mean ± standard deviation for >20 larvae. Error bars represent S.D. n.s. indicates no significant difference.