Effects of 7 days of AF4 pre-dosing on anti-apoptotic gene expression in the hippocampus following HI.

<p>Four groups of mice were pre-dosed with vehicle (Veh, 10 ml/kg/day for 7 days) or AF4 (AF4, 25 mg/kg/day for 7 days) and subjected to sham treatment (Sham; Veh-Sham, AF4-Sham) or unilateral forebrain hypoxia-ischemia (HI) (Veh-HI, AF4-HI) (A–D). The ipsilateral dorsal hippocampus was harvested 6 hours later. Fold increases in mRNAs encoding Bcl-2, cIAP1, cIAP2 and XIAP were determined by qRT-PCR, n = 6 for each group. A,*p<0.05 relative to all other groups. C and D, *p<0.05 relative to Veh-Sham and AF4-Sham. No other comparisons were significantly different. AVONA followed by Bonferroni tests.