Effects of 7 days of AF4 pre-dosing on erythropoietin gene expression in the hippocampus and striatum 1 or 6 h following HI.

<p>Four groups of mice were pre-dosed with vehicle (Veh, 10 ml/kg/day for 7 days) or AF4 (AF4, 25 mg/kg/day for 7 days) and subjected to sham treatment (Sham; Veh-Sham, AF4-Sham) or unilateral forebrain hypoxia-ischemia (HI) (Veh-HI, AF4-HI) (A–D). The ipsilateral dorsal hippocampus (A and B) and striatum (C and D) were harvested from these animals 1 h (A and C) or 6 h (B and D) later. Fold increases in mRNAs encoding erythropoietin (EPO) were determined by qRT-PCR, n = 6 for each group. B and D, *p<0.05 relative to all other groups. ^★p<0.05 relative to all other groups. No other comparisons were significantly different. AVONA followed by Bonferroni tests.