Western blot and PCR analyses of TRPV4 protein in the CA1 region of the hippocampus after hypoxia/ischemia.

<p>(<b>A</b>) Time-dependent changes in the expression of TRPV4, GFAP and β-actin proteins in the CA1 region of the hippocampus of sham-operated rats (CTRL), 1 hour (1H), 6 hours (6H), 1 day (1D), 3 days (3D) and 7 days (7D) after hypoxia/ischemia. Note that the expression of GFAP gradually increased 3 and 7 days after ischemia. β-actin was used as a loading control. (<b>B</b>) Time-dependent changes in TRPV4 protein levels showing the significant increase in TRPV4 protein 1H after H/I. Data were obtained from 3 independent Western blot analyses. (<b>C</b>) Quantitative RT-PCR revealing significantly increased TRPV4 mRNA levels 1H after H/I compared to sham-operated rats. Data were obtained from 3 independent isolations of total mRNA from the hippocampal CA1 region. Statistical significance was calculated using one-way ANOVA and Dunnett’s multiple comparison test; *p<0.05 significant, **p<0.01 very significant.