Dose-dependent reductions of HI-induced striatal neuron loss produced by oral administration of AF4.

<p>Five groups of adult male C57Bl/6 mice received either water (10 ml/kg, p.o.) or AF4 (5, 10, 25 or 50 mg/kg, p.o.) once daily for 3 days followed by 50 min of unilateral forebrain hypoxia-ischemia (HI) (left hemisphere, panels A, C, E, G, I) 24 h after the last dose. Animals were killed 2 weeks later and brain sections processed for immunohistochemical detection of the neuron specific marker NeuN. Cell counts revealed that neuroprotection was achieved by the 25 mg/kg dosing regime of AF4 and that increasing the dose of AF4 to 50 mg/kg did not produce a further reduction in neuronal loss in this structure (F). *p<0.05 versus vehicle and AF4 (5 mg/kg). No other comparisons were significantly different. AVONA followed by Bonferroni tests.