<i>daf-10</i> mutations alter the worm's response to pathogens.

<p>A. qRT-PCR analysis was performed to determine how five pathogen (PA14)-responsive genes were regulated in <i>daf-10(m79)</i> mutants in the presence of <i>E. coli</i> OP50, the normal laboratory food, or of pathogenic <i>P. aeruginosa</i> PA14 (big lawn). B–D. <i>daf-10(m79)</i> animals showed opposite phenotypes when fed <i>P. aeruginosa</i> in a small lawn assay (B) or in a big lawn assay (C). When the two assays were done in parallel (D), the survival of <i>daf-10(m79)</i> animals was similar in both conditions. In contrast, the survival of wild-type animals was dramatically reduced when a big lawn was used. A summary of the data presented in these panels and additional repeats is included in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003133#pgen.1003133.s007" target="_blank">Table S2</a>. E–F. Whereas wild-type animals avoided PA14, <i>daf-10(m79)</i> animals occupied lawns of <i>E. coli</i> OP50 and <i>P. aeruginosa</i> PA14 bacteria to a similar degree. Approximately 100 wild-type and <i>daf-10(m79)</i> worms were placed on lawns of PA14 and OP50 and imaged after 16 hrs (E). The % of worms that occupied (% occupants) the bacterial lawn was also determined (F). G–H. Representative pictures of the fluorescence signal from GFP-labeled PA14 bacteria in the intestine of worms (G) and quantitation of fluorescence signal (n≥26 worms per strain/condition) (H), showing the amount of PA14 ingested in small vs. big lawn assays by wild-type and <i>daf-10(m79)</i> worms. Error bars represent s.e.m. (^n.s.<i>p</i>>0.05, * <i>p</i><0.05, ** <i>p</i><0.01, Student's <i>t-</i>test).