Exposure to <i>P</i>. <i>aeruginosa</i> prompts gene expression changes.

(A) Schematic of experimental design. Yellow bacterial lawn indicates E. coli OP50, green bacterial lawn indicates P. aeruginosa PA14. PMK-1 and ATF-7 activation states are indicated below each condition. (B) Volcano plot of transcripts corresponding to differentially expressed protein-coding genes by exposure to P. aeruginosa PA14 versus E. coli OP50 in N2 animals. Orange and blue colored dots denote the top 100 outliers that are increased or decreased, respectively, and are annotated with their gene names. (C) Top GO terms and InterPRO classifications of transcripts that are significantly upregulated (adjusted p-value (D) Average expression (RPM) across the gene body of genes that are two-fold upregulated by exposure to pathogenic PA14. (E) Venn diagram of induced genes that are dependent upon pmk-1 or atf-7 for complete (i.e. greater than two-fold, adjusted p-value < 0.05) upregulation by PA14.