The expression levels of RPW-24–induced putative immune effectors are reduced in <i>pmk-1(km25)</i> mutants compared to wild-type animals.

<p>Six putative <i>C. elegans</i> immune effectors were analyzed by qRT-PCR in wild-type and <i>pmk-1(km25)</i> feeding on <i>E. coli</i> and exposed to either 70 µM RPW-24 or DMSO for 16 hours. Data are the average of two biological replicates each normalized to a control gene with error bars representing SEM. In (A), the data are relative to the average expression of the indicated gene in wild-type <i>C. elegans</i> exposed to DMSO. In (B), the fold change of the indicated gene above its expression in <i>C. elegans</i> exposed to DMSO is compared in wild-type and <i>pmk-1(km25)</i> mutant animals. *<i>P</i><0.01, **<i>P</i> = 0.06, ***<i>P</i> = 0.09 for the comparison in (A) of relative expression levels in wild-type versus <i>pmk-1(km25)</i> animals, each exposed to RPW-24 and in (B) for the fold change in wild-type versus <i>pmk-1(km25)</i> mutant animals.